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master's thesis
Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa
2020. urn:nbn:hr:152:336844
Vlahović, Nika
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Vlahović, N. (2020). Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:336844

Vlahović, Nika. "Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:336844

Vlahović, Nika. "Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:336844

Vlahović, N. (2020). 'Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:336844

Vlahović N. Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:152:336844

N. Vlahović, "Utjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:336844

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TitleUtjecaj visokog prehrambenog unosa kuhinjske soli na biljege upale kod TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) kontrolnih miševa
Title (english)The effect of high dietary kitchen salt intake on the markers of inflammation in TFF3-/-/C57BL/6N knockout i WT (C57BL/6N) control mice
AuthorNika Vlahović
MentorInes Drenjančević (mentor)
Committee memberIvana Jukić (predsjednik povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Physiology
Abstract
Cilj istraživanja: Cilj je studije ispitati učinak visokog unosa soli na razinu markera upale i utvrditi postoji li razlika između divljeg tipa i Tff3-/- knockout miševa. Nacrt studije: eksperimentalna studija na pokusnim životinjama Pokusne životinje i metode: Tff3-/- knockout miševi i WT kontrolni miševi (N = 6-8 po grupi) održavani su na sedmodnevnoj standardnoj prehrani glodavaca (0,4 % NaCl; niska sol; NS grupa) ili visokoslanoj (VS) prehrani (4 % NaCl). Krv je prikupljena za analizu 8 upalnih citokina (IL-lα, IL-6, IL-10, IL-17A, MCP-1, MIP-lα, MIP-l, TNF-α) u uzorcima seruma uporabom ProcartaPlex Multiplex Immunoassaya. Rezultati: Serumska koncentracija IL-6 bila je veća kod Tff3-/- miševa na NS dijeti u usporedbi s WT na NS dijeti. Nisu pronađene značajne razlike u drugim skupinama ili analitima. Nije bilo značajnih efekata visokoslane prehrane ni u jednom soju miševa. Zaključak: Visokoslana dijeta nije utjecala na proizvodnju upalnih markera u serumu. Iako se zna da TFF peptidi imaju različite funkcije, rezultati ovog istraživanja na miševima koji su knockout za Tff3-/- pokazuju da ne postoji povezanost između njihove uloge u upalnom odgovoru uzrokovanom prehranom s velikom soli.
Abstract (english)
Objective: The aim of this study was to determine the effect of high salt intake on the level of inflammation markers and to determine whether there is a difference between the wild type and the Tff3-/- knockout mice. Study Design: experimental study on animals Participants and methods: Tff3-/- knockout mice and WT control mice (N=6-8 per group) were maintained on 7 day standard rodent chow (0.4 % NaCl; low salt; LS group) or high salt (HS) diet (4 % NaCl). Blood was collected for the analysis of 8 inflammatory cytokines (IL-1α, IL-6, IL-10, IL-17A, MCP-1, MIP-1α, MIP-1β, TNF-α) in serum samples of using ProcartaPlex Multiplex Immunoassay. Results: Results: Serum concentration of IL-6 was higher in Tff3-/- mice on LS diet compared to WT on LS diet. No significant difference was found in other groups or analytes. There were no significant effects on HS diet in either strain of mice. Conclusion: HS diet has not affected the production of inflammatory markers in serum. Although TFF peptides are known to have various functions, the results of this study on Tff3 knockout mice indicates that there is no connection between their role in inflammatory response caused by high salt diet.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:336844
Study programmeTitle: University Graduate Studies
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-10-11 07:44:23