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master's thesis
O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida
2021. urn:nbn:hr:152:881940
Janić, Ivana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Janić, I. (2021). O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:881940

Janić, Ivana. "O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:881940

Janić, Ivana. "O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:881940

Janić, I. (2021). 'O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:881940

Janić I. O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:152:881940

I. Janić, "O staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:881940

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Metadata
TitleO staničnom tipu ovisna modulacija staničnog ciklusa djelovanjem 7- klorkinolin-benzoimidazolnih hibrida
Title (english)Cell specific dependent modulation of cell cycle by 7-chloroquinolinebenzoimidazole hybrids
AuthorIvana Janić
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberKatarina Mišković Špoljarić (predsjednik povjerenstva)
Committee memberMarijana Leventić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Uvod: Mnogi protutumorski lijekovi svoju aktivnost zasnivaju na zaustavljanju napredovanja stanice kroz stanični ciklus. Kako bismo stekli uvid u mehanizam djelovanja novosintetiziranih 7-klorkinolin-benzoimidazolnih hibrida ispitivan je njihov utjecaj na proliferaciju odabranih tumorskih stanica. Hipoteza: Novosintetizirani hibridi 7-klorkinolin-benzoimidazoli dovode do zaustavljanja stanica u određenoj fazi staničnog ciklusa Cilj: Cilj istraživanja je odrediti promjene staničnog ciklusa u ovisnosti o staničnom tipu tretiranih stanica 7-klorkinolin-benzoimidazolnim hibridima. Materijali i metode: U pokusu su korištene stanične linije limfoma (HuT-78) i leukemije (THP-1) te derivati 7-klorkinolin-benzoimidazola. Stanice su uzgojene u in vitro uvjetima te tretirane ispitivanim hibridima tijekom 24 sata. Nakon inkubacije, stanice su fiksirane i spremljene na -20°C do analize. Protočnom citometrijom određena je distribucija stanica po fazama staničnog ciklusa. Rezultati: Derivati KB1-KB5 doveli su do značajne promjene distribucije HuT-78 stanica, dok su derivati KB4, KB5 i KB7 značajno promijenili distribuciju THP-1 stanica. Derivat KB6 ni u jednoj staničnoj liniji nije značajno utjecao na stanični ciklus. Zaključak: Većina testiranih derivata dovodi do promjenjene distribucije stanica unutar staničnog ciklusa. HuT-78 stanice bolje reagiraju na terapiju hibridima.
Abstract (english)
Introduction: Many anticancer drugs their activity base on stopping cell progression through the cell cycle. In order to get insight into the mechanism of action of newly synthesized 7- chloroquinoline-benzoimidazole hybrids on the suppression of tumor cell proliferation, it is necessary to examine the influence of derivatives on cell cycle distribution. Hypothesis: Newly synthesized 7-chloroquinoline-benzoimidazole hybrids lead to cell arrest at a certain stage of the cell cycle. Research objectives: The aim of the study is to determine the changes in the cell cycle depending on the cell type treated with 7-chloroquinoline-benzoimidazole hybrids. Materials and methods: Lymphoma (HuT-78) and leukemia (THP-1) cell lines and 7- chloroquinoline-benzoimidazole derivatives were used in the experiment. Cells were grown in vitro and treated with derivatives for a period of 24 hours. After incubation, the cells were fixed and stored at -20°C until analysis. Distribution of cells throughphases of cell cycle we determined by flow cytometry. Results: KB1-KB5 derivatives led to a significant change in the distribution of HuT-78 cells, while KB4, KB5 and KB7 derivatives significantly changed the distribution of THP-1 cells. The KB6 derivative did not significantly affect the cell cycle in any cell line. Conclusion: Most derivatives lead to a change in cell distribution within the cell cycle. HuT- 78 cells respond better to hybrid therapy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:881940
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-12-21 12:25:39