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undergraduate thesis
Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani
2024. urn:nbn:hr:152:557036
Ćirić, Filip
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Ćirić, F. (2024). Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:557036

Ćirić, Filip. "Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:557036

Ćirić, Filip. "Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:557036

Ćirić, F. (2024). 'Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:557036

Ćirić F. Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2024 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:152:557036

F. Ćirić, "Izražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:152:557036

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TitleIzražaj gena antioksidativnih enzima u krvnim žilama Sprague-Dawley štakora na visokoslanoj i karnozinskoj prehrani
Title (english)The expression of antioxidative enzymes genes in blood vessels of Sprague-Dawley rats on high salt and carnosine diet
AuthorFilip Ćirić
MentorInes Drenjančević (mentor)
Committee memberAna Stupin (predsjednik povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2024-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Odrediti utjecaj prehrane s visokim unosom soli i nadomjestkom karnozina na izražaj gena antioksidativnih enzima u krvnim žilama kod Sprague-Dawley štakora. Materijal i metode: Zdravi Sprague-Dawley štakori starosti 8 - 10 tjedana koji su podijeljeni u četiri skupine: CTRL (kontrolna skupina koja nije izložena niti prehrani s visokim unosom soli niti nadomjestku karnozina, HSD (skupina koja je izložena prehrani s visokim unosom soli), CTRL + CAR (skupina koja je tijekom 7 dana primala oralni nadomjestak karnozina 150 mg/kg), HSD + CAR (skupina koja je tijekom 7 dana bila izložena prehrani s visokim unosom soli i kojoj je jednom dnevno dan nadomjestak karnozina). Relativni izražaj gena određen je RTqPCR metodom na Bio-Rad Real Time PCR CFX96 uređaju. Rezultati: Nakon provođenja istraživanja rezultati su pokazali da je razina genskog izražaja SOD2, SOD3,GPx1, i GPx4 i CAT povišena u HSD + CAR skupini. Izražaj gena SOD3, GPx1, i GPx4 također je povišen u CTRL + CAR skupini u odnosu na CTRL skupinu. Zaključak: Na temelju prikupljenih podataka može se zaključiti da karnozin značajno povećava izražaj gena za antioksidativne enzime, osobito u uvjetima visokog unosa soli. Ovo sugerira da karnozin ne samo da neutralizira slobodne radikale, već i potiče aktivnost gena odgovornih za proizvodnju enzima koji štite stanice od oksidativnog stresa, pružajući dodatnu zaštitu u takvim uvjetima.
Abstract (english)
Objectives: To investigate the influence of a high salt diet and carnosine supplementation on gene expression of antioxidative enzymes in the blood vessels of Sprague-Dawley rats. Material and Methods: Healthy Sprague-Dawley rats aged 8 - 10 weeks that were divided into four groups: CTRL (control group exposed to neither high salt diet nor carnosine supplement), HSD (group exposed to a high salt diet), CTRL + CAR (group that received 150mg/kg oral carnosine supplementation for 7 days), HSD + CAR (group that was exposed to a high salt diet for 7 days and was given a carnosine supplement once a day). Relative gene expression was determined by the RTqPCR method on a Bio-Rad Real Time PCR CFX96 device. Results: After conducting the research, the results showed that the gene expression levels of SOD2, SOD3, GPx1, GPx4, and CAT were elevated in the HSD + CAR group. The expression of SOD3, GPx1, and GPx4 genes was also elevated in the CTRL + CAR group compared to the CTRL group. Conclusion: Based on the collected data, it can be concluded that carnosine significantly increases the expression of antioxidant enzyme genes, particularly under conditions of high salt intake. This suggests that carnosine not only neutralizes free radicals, but also stimulates the activity of genes responsible for producing enzymes that protect cells from oxidative stress, thereby providing additional protection under such conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:557036
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2024-11-08 10:34:36