Objective of the Study: The aim of this research is to determine the activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in the serum of Sprague - Dawley rats following a high-salt diet with and without oral supplementation of carnosine. Study Design: Experimental study on the tissues of test animals (Sprague - Dawley rats). Materials and Methods: Samples were collected by randomly dividing healthy SpragueDawley rats of both sexes into four groups: control group (CTRL, not exposed to either carnosine or high-salt diet), HSD group (exposed to a high-salt diet), CTRL + CAR group (group given oral carnosine supplementation), and HSD + CAR group (exposed to a high-salt diet and given carnosine supplementation). Prior to sampling, rats were anesthetized. After the onset of anesthesia, they were euthanized by decapitation, and blood was collected into tubes without anticoagulants. The collected blood was centrifuged, the supernatant (serum) was separated and stored at -80ºC until enzyme activity measurements. The activity of antioxidant enzymes — superoxide dismutase, catalase, and glutathione peroxidase — was measured spectrophotometrically using a spectrophotometer with kinetic options. Results: Adding carnosine did not significantly affect catalase activity, although a slight increase was observed in the high-salt group. On the other hand, high salt intake significantly increased glutathione peroxidase activity, and carnosine further enhanced this response. Additionally, high salt intake decreased superoxide dismutase activity, while carnosine significantly increased this activity, especially under high salt intake conditions. Conclusion: High salt intake significantly impairs the oxidative status, while carnosine effectively enhances the antioxidant response, particularly under high salt intake conditions, highlighting its importance in maintaining antioxidant balance.