Objective: To determine how nutrition of mothers during pregnancy and lactation, and the male offspring of rats at different regime of diet can affect body weight, body mass index, the concentration of inflammatory cytokines in the blood and the intensity of immunohistochemistry staining of TNF-alpha in bone tissue and in the liver. Furthermore, how high-fat diet can cause fat infiltration in the bone marrow and in the liver, which can additionally cause change of volume and structure of bone tissue of the fifth lumbar vertebra. Methods: Ten female Sprague Dawley rats were at nine weeks of age randomly divided in two groups and fed either a control diet or food rich in saturated fatty acids during six weeks, and then mated with genetically similar male subjects. After gestation and lactation, male offspring from both groups were divided in four subgroups depending on the diet they were fed until 22 weeks of age. Body weight throughout the experiment was measured and body mass index was calculated, organ weight was valued after sacrifice, and total cholesterol, triglyceride, AST, ALT, IL-6 and TNF-alpha in serum were measured. Bone microstructure was analyzed in the fifth lumbar vertebra using digital photographic images. TNF-alpha expression was measured in bone and liver samples using immunohistochemistry. Results: Body weight was lower in offspring fed a diet rich in fats and the analysis of organ weight showed greater differences in offspring with changed intrauterine/postnatal feeding regime compared with the control group. In all offspring fed high-fat diet higher serum cholesterol and triglyceride levels were observed compared to the control group while the offspring that had their diet replaced in relation to mothers' diet (CD-HFD, HFD-CD) recorded higher concentrations of inflammatory cytokines in the blood. The offspring of healthy mothers, fed with high-fat diet, showed increased accumulation of fat in the bone marrow and disturbed trabecular bone structure and the highest intensity of TNF-alpha immunohistochemical staining of bone marrow and liver tissue, and also worse histopathological analysis of liver parenchyma. Conclusion: The group of rats whose mother was fed with controlled laboratory chow and then offspring with high-fat diet had higher serum concentration of inflammatory cytokines in and the highest level of immunohistochemical signal of TNF-alpha in the fifth lumbar vertebra and liver parenchyma. Thus we can conclude that additional accumulation of body fat because of eating a diet with high percentage of fat stimulates the accumulation of fat in the bone marrow and liver, which can encourage the inflammatory response by stimulating cytokines, here observed TNF-alpha, which is subsequently involved in the pathogenesis of bone remodeling and development of NAFLD.