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Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus
2017. urn:nbn:hr:152:757778
Živković, Valentina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Živković, V. (2017). Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:757778

Živković, Valentina. "Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:757778

Živković, Valentina. "Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:757778

Živković, V. (2017). 'Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:757778

Živković V. Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:152:757778

V. Živković, "Ispitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:757778

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TitleIspitivanje sposobnosti stvaranja biofilma kod kliničkih izolata Pseudomonas aeruginosa i Staphylococcus aureus
Title (english)Investigation of biofilm formation ability of Pseudomonas aeruginosa and Staphylococcus aureus clinical isolates
AuthorValentina Živković
MentorDomagoj Drenjančević (mentor)
Committee memberSuzana Mimica-Matanović (predsjednik povjerenstva)
Committee memberJerko Barbić (član povjerenstva)
Committee memberDomagoj Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2017-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Cilj istraživanja: Svrha ovog istraživanja bila je ispitati sposobnost formiranja biofilma kliničkih izolata Staphylococcus aureus i Pseudomonas aeruginosa na dvama različitim bujonima, Mueller-Hunton i Luria-Bertani, usporediti sposobnost stvaranja biofilma između dviju navedenih bakterijskih vrsta te usporediti sposobnost detekcije biofilma s obzirom na in vitro uvjete, odnosno bujone. Materijali i metode: U istraživanju je korišteno 30 sojeva Pseudomonas aeruginosa i 30 sojeva Staphylococcus aureus dobivenih iz kliničkih uzoraka. Nakon pripreme, suspenzije bakterija inokuliranih na bujone nasađene su na mikrotitarske pločice, izmjerena je produkcija biofilma na spektrofotometrijskom čitaču na 550 nm te su rezultati dobiveni pomoću formula klasificirani u četiri kategorije. Rezultati: Rezultati su pokazali da oba ispitivana soja, uspješno i statistički značajno, stvaraju biofilm u oba navedena medija za uzgoj. Iz navedenih rezultata može se iščitati da je za gram negativni soj P. aeruginosa na oba bujona najveći postotak pojavnosti imala jaka produkcija, dok se kod S. aureus jaka produkcija pojavila u samo jednom mjerenju. Nadalje, nema statistički značajne razlike u stvaranju biofilma između sojeva ni na jednom ni na drugom korištenom bujonu, a isto se pokazalo i pri usporedbi stvaranja između bujona i kod jedne i kod druge bakterije. Zaključak: Rezultati ovog istraživanja neizravno upućuju na poznate spoznaje o patogenosti i virulenciji ovih uzročnika i mogu doprinijeti boljem razumijevanju njihovih in vivo osobina u izazivanju infekcija povezanih s biofilmom.
Abstract (english)
Objectives: The goal of this investigation is to examine the ability of forming biofilm in clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa on two different broths - Meuller-Hinton and Luria-Bertani, as well as to compare the ability to form biofilm among the two mentioned types of bacteria and the ability of detecting the biofilm considering in vitro conditions, that is broths. Material and methods: 30 strains of Pseudomonas aeruginosa and 30 strains of Staphilococcus aureus obtained from clinical specimens were used for this investigation. After preparing the suspensions of bacteria inoculated on broths, they were set on microtiter plates and the biofilm production was measured using the spectrophotometric reader on 550 nm. The final results based on the formulas were classified into four categories. Results: The results showed that both of the tested strains, successfully and statistically significant, form a biofilm in both of the above mentioned broths. The results clearly indicate that for the gram negative P.aeruginosa strain in both broths the highest percentage of occurrence had a strong production, whereas in S.aureus a strong production appeared only in one measurement. Furthermore, there is no statistically significant difference in the biofilm formation between the strains on neither of the used strain and the same results occured after comparing the formation between the broths in both of the bacteria. Conclusion: The results of this investigation indirectly point to known cognitions of pathogenicity and virulence of these agents and can contribute to the better understanding of their in vivo characteristics in causing of infections related to biofilm.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:757778
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-04-12 12:23:49