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undergraduate thesis
Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom
2018. urn:nbn:hr:152:094879
Ganjto, Marija
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Cite this document

Ganjto, M. (2018). Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:094879

Ganjto, Marija. "Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:094879

Ganjto, Marija. "Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:094879

Ganjto, M. (2018). 'Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 03 April 2025, https://urn.nsk.hr/urn:nbn:hr:152:094879

Ganjto M. Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2025 April 03] Available at: https://urn.nsk.hr/urn:nbn:hr:152:094879

M. Ganjto, "Sastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:094879

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TitleSastav lipida stanične membrane jetre mužjaka štakora Sprague Dawley hranjenih masnom i slatkom hranom i tretiranih metforminom ili liraglutidom
Title (english)Liver plasma membrane lipid coposition in Sprague Dawley male rats fed with fatty and sweet food and treated with metformin or liraglutide
AuthorMarija Ganjto
MentorMarija Heffer (mentor)
Committee memberInes Drenjančević (predsjednik povjerenstva)
Committee memberMartina Mihalj (član povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Postoji pretpostavka da loša prehrana izravno utječe na promjene u sastavu stanične membrane. Osim toga primijećeno je kako dijabetične životinje imaju smanjen broj gangliozida u staničnim membranama što dovodi do pretpostavke da smanjen broj gangliozida izravno utječe na razvoj dijabetesa tip 2. Kako neki od postojećih antidijabetika mogu utjecati na sastav stanične membrane ispitan je njihov utjecaj. Cilj: Usporediti sastav i količinu gangliozida stanične membrane jetre mužjaka... More Sprague Dawley na masnoj i slatkoj dijeti te životinja koje su uz takvu prehranu dobivale i antidijabetike metformin i liraglutid. Materijal i metode: Istraživanje je provedeno na tkivu 12 nasumično odabranih životinja, po 3 životinje iz svake skupine. Izolacija gangliozida iz jetrenog tkiva napravljena je Svennerholmovom metodom organskim otapalima, dok je za njihovo razdvajanje korištena tankoslojna kromatografija (TLC) na silika-gel pločicama visoke sposobnosti razlučivanja (HPTLC). Rezultati: Prehrana bogata mastima i šećerom uzrokuje smanjenje gangliozida b i c serije, kao i GM3, dok dolazi do povećanja količine X gangliozida. Učinak metformina je obrnut, tj. dolazi do povećanja većine gangliozida, posebno GM3. Sam liraglutid djeluje tako da smanjuje količinu svih gangliozida, dok u kombinaciji sa hranom bogatom mastima i šećerom dolazi do povećanja samo GM3. Zaključak: Masna i slatka prehrana utječe na smanjenje ekspresije gangliozida staničnih membrana i samim time mijenja sastav stanične membrane. Nadalje, antidijabetici su pokazali različit utjecaj na povećanje, odnosno smanjenje ekspresije gangliozida u staničnim membranama. Less
Abstract (english)
Liver plasma membrane lipid coposition in Sprague Dawley male rats fed with fatty and sweet food and treated with metformin or liraglutide Introduction: There is assumption that bad food consumation affects alternations in composition of cell membrane. Additionaly, animals which have diabetes also have decreased number of gangliosides what leads to assumption that decreased number of gangliosides directly affects development of diabetes type 2. Since some of antidiabetics can affect... More composition of cell membrane their influence was examined. Aim: Purpose of this study was to compare composition and quantity of gangliosides in liver cell membrane in Sprague Dawley male rats fed with fatty and sweet food and animals that have been given antidiabetics metformin and liraglutide along with such diet. Materials and methods: Research was carried out on tissue of 12 randomly selected animals, 3 animals from each group (males on standard diet, males fed with fatty and sweet food, males fed with fatty and sweet food who got metformin along with their diet and males fed with fatty and sweet food who got liraglutide along with their diet). Extraction of gangliosides from liver tissue was made by Svennerholm's method of organic solvents, while for their separation was used Thin-Layer Chromatography (TLC) on High Performance Thin-Layer Chromatography (HPTLC) silica-gel tiles. Results: Fatty and sweet food causes decrease in gangliosides of b and c series, so as in GM3 ganglioside, but it increases quantity of X ganglioside. Effect of metformin is reversed here, respectively metformin increases majority of gangliosides, especially GM3. Liraglutide decreases quantity of all gangliosides, while in combination with fatty and sweet food liraglutide increases only GM3. Conclusion: Fatty and sweet food affect the reduction of ganglioside expresion in cell membranes and thus change composition of cell membrane. Furthermore, antidiabetics have shown different influences on the increase or decrease in the expresion of gangliosides in cell membranes. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:094879
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-12-13 13:30:43
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