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undergraduate thesis
Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora
2018. urn:nbn:hr:152:546576
Papak, Zdenka
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Papak, Z. (2018). Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:546576

Papak, Zdenka. "Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:546576

Papak, Zdenka. "Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2018. https://urn.nsk.hr/urn:nbn:hr:152:546576

Papak, Z. (2018). 'Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:546576

Papak Z. Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2018 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:546576

Z. Papak, "Utjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:152:546576

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TitleUtjecaj primjene blokatora angiotenzin II AT-1 receptora (losartana) na izražaj proteina ciklooksigenaze I i II u krvnim žilama Sprague-Dawley štakora
Title (english)The effect of blocade of angiotensin II AT-1 receptors (losartan) on the protein expression of cyclooxygenase I and II in blood vessels of Sprague-Dawley rats
AuthorZdenka Papak
MentorInes Drenjančević (mentor)
Committee memberMartina Mihalj (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2018-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Cilj: Utvrditi izražajnost proteina enzima ciklooksigenaze 1 i ciklooksigenaze 2 u krvnim žilama štakora koji su tjedan dana pili vodu s losartanom (blokatorom angiotenzin II AT-1 receptora) na modelu zdravih Sprague-Dawley štakora pomoću molekularne metode Western blot. Materijali i metode: Zdravi muški Sprague-Dawley (SD) štakori u dobi 9 - 11 tjedana starosti. Jedna je skupina štakora pila običnu vodu (CTRL skupina, kontrolna), a druga je skupina štakora kroz vodu za piće primala 40 mg losartana (ukupna dnevna doza) kroz sedam dana (CTRL+LOSARTAN skupina). U uzorcima krvnih žila mozga Western blot metodom, određen je izražaj proteina ciklooksigenaze 1 (COX-1) i ciklooksigenaze 2 (COX-2). Rezultati: Izražaj COX-2 statistički je značajno povećan u CTRL+LOSARTAN skupini u usporedbi s CTRL skupinom. Razlika izražaja COX-1 u CTRL-LOSARTAN skupini u odnosu na CTRL skupinu nije statistički značajna. Zaključak: Primjena blokatora angiotenzin II AT-1 receptora (losartana) tijekom sedam dana uzrokuje značajnije povećanje u izražaju enzima ciklooksigenaza 2 (COX-2) u moždanim krvnim žilama Sprague-Dawley štakora. U izražaju enzima ciklooksigenaza 1 (COX-1), za razliku od COX-2, nema značajnije promjene.
Abstract (english)
Aim: To determine the expression of the enzyme cyclooxygenase 1 and cyclooxygenase 2 protein in the blood vessels of the rats that were weekly drinking water with losartan (angiotensin II receptor AT-1 blocker) on a model of healthy Sprague-Dawley rats using a Western blot molecular method. Materials and Methods: Healthy male Sprague-Dawley (SD) rats were 9-11 weeks old. One group of rats was drinking common water (CTRL group, control), and the other group of rats received 40 mg of losartan (total daily dose) through water for seven days (CTRL + LOSARTAN group). The expression of cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) proteins in brain blood vessel samples was determined by the usage of the Western blot method. Results: Expression of COX-2 was statistically significantly increased in the CTRL + LOSARTAN group compared to the CTRL group. And the difference in expression of COX-1 in the CTRL-LOSARTAN group compared to the CTRL group is not statistically significant. Conclusion: Inatake of angiotensin II AT-1 receptor blocker (losartan) in organism for seven days causes a significant increase in the expression of cyclooxygenase 2 (COX-2) enzyme in the brain blood vessels of Sprague-Dawley rats. There are no significant changes in expression of enzyme cyclooxygenase 1 (COX-1).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:546576
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-12-14 09:03:14