Objectives: The aim of the study was to compare the surface area of adipocytes, the amount of mitochondria, lipid droplets and collagen in the adipose tissues of two age groups (6 and 14.5 months) of female Sprague Dawley rats that were exposed to chronic or sham stress. Study design: The study was organized as a study of couples. Female rats exposed to chronic stress were statistically compared to females exposed to sham stress. Also, young and old females were compared statistically. Materials and methods: The study used three types of adipose tissues – mesenteric, subcutaneous and interscapular adipose tissue – collected from a total of 39 animals (19 young female rats - 9 young females exposed to chronic stress and 10 young control animals; 20 old female rats - 10 old females exposed to chronic stress and 10 old control animals). Tissues were stained with three histological stains (hemalaun / eosin, hematoxylin phosphotungstic acid, and Picrosirius red) and quantified in ImageJ (FIJI) software. Results: Exposure to chronic stress in older animals significantly affected the increase in adipocytes surface area, while in young animals the cell volume remained unchanged. Aging caused the decrease of mitochondria content, and increase of lipid droplets content in the older female animal group. The content of collagen in brown and white adipose tissues increased in older females as an effect of aging. Moreover, collagen content increased in white adipose tissue as an effect of stress in both age groups. Conclusion: Chronic stress affects the volume of adipocytes of old animals. The amount of mitochondria and lipid droplets is age dependent. With age, the content of mitochondria decreases and the content of fat droplets increases. The increase in collagen content depends on a combination of aging and chronic stress.