OBJECTIVES: The aim of this study is to show laboratory diagnostics and frequency of enterobiosis and its frequency in the area of Split-Dalmatia County in the period from January 1, 2022 until December 31, 2023, and to investigate the sociodemographic characteristics of the patients, the incidence of the disease by month during the calendar year and the incidence of the disease within one household. STUDY DESIGN: A Retrospective cohort epidemiological study conducted from January 1, 2022 until December 31, 2023. PARTICIPANTS AND METHODS: 690 persons who were diagnosed with enterobiosis during the two-year period in the SDC were included in the research. The results were obtained by reviewing the archives of the Clinical Microbiology Department at the Public Health Institute in Split-Dalmatia County. All subjects were previously diagnosed with E. vermicularis infection by microscopic examination of the tape test and stool. RESULTS: Of the 690 patients, 52% were men and 48% were women. Children under the age of 18 were statistically significantly diseased than adults (97.5%). Most people diseased during autumn (33.5%), i.e. during November and December. Most of the patients lived in the city (87.5%), and 12.3% of respondents were found to have spread within the household. CONCLUSION: The analysis of the obtained results concludes that there are no statistically significant differences in the diseased incidence between the sexes. Children were statistically significantly more diseased than adults. There is no statistically significant difference between men and women in the incidence of the disease in a particular season, nor in the incidence of the disease within one household.