HPV infection is one of the most common sexually transmitted diseases. Low-risk genotypes cause anogenital warts outside the genitals, while high-risk genotypes are associated with intraepithelial neoplasia and cervical cancer. The methods of choice for the detection of HPV infection are based on methods of molecular diagnostics – PCR amplification. The study used data from the electronic database of the Department of Microbiology, County Public Health Institute of Osijek-Baranja County. In the period from 2010 to 2014 in Osijek-Baranja County, there were 2 979 tested samples, of which 1468 (49%) were positive for HPV; 538 of which were detected genotypes. In the period from 2011 to 2013, more than 50% of the total number of tested women had HPV infection. It can be concluded that women until 2014 had more HPV infection than men, but we need to consider the possibility lower number of testing and registration disease in men. Data of genotyped HPV samples show that men more often suffer from low-risk HPV infections than women, who more often suffer from high-risk HPV infections. The most common high-risk genotypes in women and men are 16 and 51. The most common low-risk genotypes in women are 6 and 84, and in men 6. The largest number of mixed infections was recorded in 2012 with 94 (64%) positive samples. Compared with other counties, there is relatively small number of vaccinated people in Osijek-Baranja County with 47 vaccinated people in the period of 9 years. Keywords: HPV, genotype, vaccination, Osijek-Baranja County