INTRODUCTION: Effective DNA isolation from various biological samples is the basis for successful forensic analysis of genetic material. Ion-exchange resin and commercial silica column are the most common examples of non-affinity and affinity methods used for DNA isolation from scarce biologic samples such as blood stains or buccal swabs. Choosing the optimal DNA isolation method is thus essential for subsequent forensic analyses, which is why the efficiency of Chelex® InstaGene ™ and Qiagen® QIAamp Mini commercial kits was examined. AIM: To determine the efficacy of the investigated methods for DNA isolation from buccal swabs and blood stains by measuring the amount and quality of extracted DNA on QUBIT fluorometer and qPCR. MATERIAL AND METHODS: Blood samples and buccal swabs were used for this study. DNA was isolated with the InstaGene reagent and the QIAamp Blood Mini kit. The DNA concentration was subsequently measured on the QUBIT fluorometer and a qPCR machine using Qubit High Sensitivity DNA and QunatiFiler Human Quantification DNA Kits. RESULTS: A significantly higher yield of DNA material was gained from buccal swabs in comparison with the amount of DNA obtained from blood stains. No substantial difference was found in the amount of DNA isolated from the same kind of samples, regardless of isolation method used. The highest degree of Lin’s concordance between measurements made on QUBIT fluorometer and the qPCR machine was recorded in buccal swab samples, with the measurement concordance declining as the yield of DNA material decreased. CONCLUSION: Compared to the InstaGene reagent, the QIAamp Blood Mini Kit is an equally good alternative for DNA isolation from buccal swabs, but is less acceptable for isolating optimal amounts of DNA from blood stains.