Objectives: The aim of this study was to examine whether there is a significant relationship between personality traits, stress, sleep quality and academic success of medical laboratory diagnostics students Study design: The study is structured as observational, cross-sectional research Participants and methods: The research included 30 undergraduate and 28 graduate students of medical laboratory diagnostics. Considering the current epidemiological situation, the pandemic of the COVID-19 virus, it was conducted through the website www.google.com, using the Google Forms tool. The questionnaire is composed of 4 parts; basic demographic data, testing the level of depression, anxiety and stress using the DASS-21 questionnaire, the IPIP-50 Big Five personality model questionnaire and the PSQI sleep quality questionnaire. Results: This research confirmed the results of the previous studies on the correlation of certain personality traits with academic success, but in this research, without a significant connection. A negative association between depression and academic success was confirmed, as well as a positive association between stress and bad sleep quality, but no association with academic success. Conclusion: Consistent with previous research, personality traits and depression are associated with academic success. The correlation between stress and sleep quality was also confirmed, but without correlation with grade point average. A large percentage ofstudents with poor sleep quality was observed, for which reason it is necessary to emphasize the importance of a proper circadian rhythm and a life with as little stress as possible, which, with good organization, lead to the fulfillment of set goals.