OBJECTIVES: The goals of this research were to measure the morphological features of adipocytes in perigonadal adipose tissue in ovariectomized rats and those that were not ovariectomized, using the Adiposoft plug-in in the Fiji software package, and to compare the given results to check whether the analysis of samples stained using the picrosirius red method would give results comparable to those stained using the hematoxylin-eosin method. Additionally, the morphology of adipocytes of ovariectomized animals was compared with those that were not ovariectomized. STUDY DESIGN: The research was conducted as a non-randomized controlled experiment on animals. MATERIALS AND METHODS: In this research, perigonadal adipose tissue of female Wistar rats was used. A total of 18 samples of perigonadal adipose tissue were used and stained using hematoxylin-eosin and picrosirius red methods. Eight samples within each staining group were classified as ovariectomized and ten as control. In the analysis, the diameter and area were measured. Volume was calculated from the diameter. The photos of the samples were processed with the Adiposoft plug-in for the Fiji software. RESULTS: Upon examining the samples under a light microscope and conducting statistical analysis of the computer-measured features, no significant difference was observed in the diameter, surface area, and volume of adipocytes of the perigonadal adipose tissue of the ovariectomized and control groups, as well as the differences in these features obtained using standard or tested staining. CONCLUSION: Morphological characteristics of adipocytes in the perigonadal adipose tissue do not differ significantly when comparing ovariectomized and control groups, nor when comparing standard and tested staining. Picrosirius red staining can be used with Adiposoft plug-in instead of hematoxylin-eosin staining.