Objectives: The main goal was to investigate if there is a correlation between vascular access and peripheral and central blood pressures, hemodynamic indicators and estimated indicators of artery rigidity. Study Design: The study is a cross-sectional study. Participants and methods: Participants are 107 patients in end stage renal disease that are being treated with maintenance hemodialysis in Renal Division, Department of Medicine, University Hospital Center Osijek, Croatia. With “Agedio B900” device we measured, in a non-invasive way, peripheral arterial pressure and pulse wave velocity. Central aortic pressure, cardiac output, peripheral resistance, augmentation pressure, augmentation index, reflection coefficient and artery rigidity were estimated via special software in the device. Results: The study did not indicate correlation between vascular access and peripheral and central arterial pressure, hemodynamic indicators (peripheral resistance, cardiac output and cardiac index; only a significantly higher pulse pressure amplification in patients with arteriovenous fistula (AVF) compared to patients with central venous catheter), nor estimated indicators of arterial rigidity (augmentation pressure, augmentation index, reflection coefficient and pulse wave velocity). Peripheral and central systolic arterial pressure were significantly lower after a dialysis session, which could be related to a significantly lower body mass and body mass index after dialysis. Conclusion: Although this study did not show it, there are other studies showing a connection between AVF and hemodynamic indicators and estimated indicators of artery rigidity. For that reason, we suggest conducting studies with different designs, which could better assess the impact of vascular access on hemodynamics and artery rigidity. We have a high prevalence of AVF (56 patients, 52.3%), but it is still lower than prevalence in the rest of Europe and in the USA.