Objectives: The aim of this study was to examine whether there is correlation between the citrate inhibitors levels with the presence of calcium stones in urine. The degree of calcium satiation in urine was determined in patients with calcium urolithiasis and compared with the control group in order to determine the inhibitory activity of the urine from urine citrate levels. Finally, the aim was to determine the influence of the risk index represented by the calcium citrate ratio on the development of urolithiasis. Study design: The study was structured as a cross-sectional study of historical data. Participants and Methods: The study included 97 respondents divided into two groups. The first group consisted of respondents with calcium urolithiasis, and the second group were respondents who did not report stone formation (control group). Variables included in the study were as follows: sex, age, pH levels, calcium and citrate levels, and the stone composition for patients suffering from urolithiasis, which was determined by infrared spectroscopy. Results: The median age of urolithiasis respondents was 50. The main component of 52 % of the analyzed stones was calcium oxalate monohydrate, which correlates to the elevated calcium urine concentration of the respondents. This fact corroborates the hypothesis that calcium satiation in urine is one of the key factors in the development of calcium stones. In the study, calcium citrate concentration ratio was considered risk factor for the development of the stones and respondents with urolithiasis were proven to have had higher risk index that the control group. Conclusion: Respondents with calcium urolithiasis have higher degree of urinary excretion of calcium, lower concentration of citrate in urine, and higher calcium citrate ratio.