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master's thesis
Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti
2023. urn:nbn:hr:152:432624
Zidar, Ana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Cite this document

Zidar, A. (2023). Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:432624

Zidar, Ana. "Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:152:432624

Zidar, Ana. "Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2023. https://urn.nsk.hr/urn:nbn:hr:152:432624

Zidar, A. (2023). 'Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 17 July 2024, https://urn.nsk.hr/urn:nbn:hr:152:432624

Zidar A. Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2023 [cited 2024 July 17] Available at: https://urn.nsk.hr/urn:nbn:hr:152:432624

A. Zidar, "Učinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:152:432624

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TitleUčinak nadomjestka karnozina na razinu oksidativnog stresa kod Sprague-Dawley štakora na visokoslanoj dijeti
Title (english)The effect of carnosine supplementation on the level of oxidative stress in Sprague-Dawley rats on a high-salt diet
AuthorAna Zidar
MentorZrinka Mihaljević (mentor)
Committee memberAna Stupin (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberZrinka Mihaljević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2023-07-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Cilj istraživanja: Ispitati učinak suplementacije karnozinom na proizvode oksidacije proteina i napredne oksidirane proteinske proizvode u serumu i tkivima Sprague-Dawley štakora prije i nakon visokog unosa soli uz suplementaciju karnozinom; razine malondialdehida, stvaranje ROSa i vrijednosti antioksidativnog kapaciteta nakon suplementacije karnozinom. Nacrt studije: Pokusno (eksperimentalno) istraživanje. Materijali i metode: Sprague-Dawley štakori bili su podijeljeni u četiriskupine: zdravi netretirani štakori, štakori koji su konzumirali hranu sa visokim udjelom kuhinjske soli, štakori koji su konzumirali uobičajenu hranu sa niskim udjelom kuhinjske soli i kroz oralnu sondu dobivali suplementaciju karnozina te štakori koji su konzumirali hranu sa visokim udjelom kuhinjske soli i kroz oralnu sondu dobivali suplementaciju karnozina. Mjerenje naprednih oksidiranih proteinskih proizvoda, razine malondialdehida i vrijednosti antioksidativnog kapaciteta nakon suplementacije karnozinom provodilo se u Laboratoriju za molekularnu i kliničku imunologiju Medicinskog fakulteta Osijek. Rezultati: Rezultati su pokazali kako je razina naprednih oksidiranih proteinskih proizvoda značajno povišena u skupini štakora koji su podvrgnuti prehrani sa visokim udjelom kuhinjske soli u usporedbi s ostalim skupinama u uzorcima seruma te smanjena nakon suplementacije karnozinom. Razina oksidativnog stresa određena mjerenjem TBARS u serumu Sprague-Dawley štakora između svih ispitivanih skupina nije se značajno razlikovala. U skupini štakora koji su podvrgnuti prehrani sa visokim udjelom kuhinjske soli utvrđena je značajna razlika u razini antioksidativnog kapaciteta u serumu u odnosu na ostale skupine. Zaključak: Rezultati potvrđuju da je povećani oksidativni stres povezan s unosom visokog udjela kuhinjske soli te da nadomjestak karnozina ima antioksidativni učinak.
Abstract (english)
Objectives: To examine the effect of carnosine supplementation on protein oxidation products and advanced oxidized protein products in serum and tissues of Sprague-Dawley rats before and after high salt intake with carnosine supplementation; malondialdehyde levels, ROS formation and antioxidant capacity values after carnosine supplementation. Study design: Experimental research Materials and Methods: Sprague-Dawley rats were divided into four groups: healthy untreated rats, rats that consumed a high-salt diet, rats that consumed a normal low-salt diet and received carnosine supplementation through an oral gavage, and rats that consumed with a high content of table salt and received carnosine supplementation through an oral tube. The measurement of advanced oxidized protein products, malondialdehyde levels and antioxidant capacity values after carnosine supplementation was carried out in the Laboratory for Molecular and Clinical Immunology of the Faculty of Medicine Osijek. Results: The results showed that the level of advanced oxidized protein products was significantly increased in the group of rats fed a high salt diet compared to other groups in serum samples and decreased after carnosine supplementation. The level of oxidative stress determined by measuring TBARS in the serum of Sprague-Dawley rats was not significantly different between all the investigated groups. In the group of rats that were subjected to a diet with a high content of table salt, a significant difference in the level of antioxidant capacity in the serum was found compared to the other groups. Conclusion: The results confirm that increased oxidative stress is associated with high salt intake and that carnosine replacement has an antioxidant effect.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:432624
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-12 07:54:04