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undergraduate thesis
Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro
2020. urn:nbn:hr:152:159958
Ruška, Matej
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

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Ruška, M. (2020). Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:159958

Ruška, Matej. "Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:159958

Ruška, Matej. "Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:159958

Ruška, M. (2020). 'Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 19 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:159958

Ruška M. Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2024 November 19] Available at: https://urn.nsk.hr/urn:nbn:hr:152:159958

M. Ruška, "Određivanje katalitičke aktivnosti salivarne alfa amilaze in vitro", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:159958

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Metadata
TitleOdređivanje katalitičke aktivnosti salivarne alfa amilaze in vitro
Title (english)Determination of the catalytic activity of salivary alpha amylase in vitro
AuthorMatej Ruška
MentorKatarina Mišković Špoljarić (mentor)
Committee memberBarbara Viljetić (predsjednik povjerenstva)
Committee memberHrvoje Brkić (član povjerenstva)
Committee memberKatarina Mišković Špoljarić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
CILJ ISTRAŽIVANJA: Odrediti aktivnost salivarne alfa amilaze proizašle iz različitih proizvodnih serija in vitro. NACRT STUDIJE: Presječno istraživanje. MATERIJALI I METODE: Analizirana je aktivnost dviju komercionalno dostupnih alfa-amilaza. Korištena alfa–amilaza (ptijalin) kupljena je od tvrtke Sigma Aldrich. Korištene su sljedeće kemikalije: škrob, D-maltoza monohidrat, 3,5-dinitrosalicilna kiselina, natrijev klorid, natrij-hidroksid i kalij-natrij-tartarat-4-hidrat. Za određivanje salivarne α–amilaze korištena je modificirana metoda po Bernfeldu koja se temelji na svojstvu maltoze da reducira 3,5-dinitrosalicilnu kiselinu i stvara narančasto–crvenu otopinu. Intenzitet promjene boje proporcionalan je nastaloj maltozi, a apsorbancija se mjeri na valnoj duljini 540 nm. REZULTATI: Neparametrijskim Mann-Whitney U testom za dva nezavisna uzorka utvrđeno je da nema statistički značajne razlike u enzimskoj aktivnosti između ovih dviju salivarnih alfa amilaza proizašlih iz različitih proizvodnih serija in vitro. ZAKLJUČAK: Provedenim istraživanjem dolazimo do zaključka da su obje serije analizirane α–amilaze jednako učinkovite te da nema značajne statističke razlike u njihovoj enzimskoj aktivnosti unatoč različitom vremenu isporuke. Metoda po Bernfeldu treba dodatnu prilagodbu kako bi se poboljšali i optimizirali reakcijski uvjeti za analizu uzoraka humane α-amilaze.
Abstract (english)
OBJECTIVES: The aim of this study was to determine salivary α–amylase activity bought from two different commercially available production batches. STUDY DESIGN: A cross sectional study. MATERIAL AND METHODS: Salivary α–amylase activity was determined from two commercionally bought samples. α–amylase (ptijalin) was baught from Sigma Aldrich. The following solutions were made: sodium phosphate buffer, starch solution, DNS reagent and maltose standard by using these chemicals : starch, D–maltose monohydrate, 3,5,-dinitrosalicylic acid, sodium chloride, sodium hydroxid and potassium sodium tartrate 4–hydrate. For the determination of salivary α–amylase activity modified Bernfeld method was used. This method is based on the properties of maltose to reduce 3,5-dinitrosalicylic acid into the orange–red solution. The intensity of the colour is proportional to the maltose released. RESULTS: Using nonparametric Mann-Whitney U test for two independent samples it was determined that there is no statistically significant difference in the enzymatic activity between these two samples. CONCLUSION: The conducted research leads to the conclusion that there is no statistically significant difference in enzymatic activity between two different commercially available production batches despite different delivery times. Bernfeld method needs additional adjustment to improve and optimize reaction conditions for analysis of human α–amylase.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:159958
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-11-12 12:10:03